Besides spatial shading effects, time-lapse movies often exhibit a temporal baseline drift due to background bleaching, which further skews the quantification of the dynamic behaviour of cellular and molecular properties 2. This not only degrades the visual quality of an image (for example, by causing discontinuities in whole slide images (WSIs)), but more critically compromises the downstream analysis of, for example, tissue composition or single-cell properties. However, optical microscopy data, and especially fluorescence imaging, is often severely affected by shading or vignetting 1, typically reflected as an attenuation of the brightness intensity from the centre of the optical axis to the edges. All modern optical imaging (whether whole slide imaging, high-content screening or high-throughput time-lapse microscopy) relies on image processing and quantification methods to analyse and interpret the acquired data. Optical imaging is an indispensable tool in biomedical research.
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